1222 TRAF6 regulates autophagy and apoptosis of melanoma cells through c-Jun/ATG16L2 signaling pathway
نویسندگان
چکیده
Both autophagy and apoptosis are essential processes participating in cell death maintain cellular homeostasis. The dysregulation of these two biological results development diseases including cancers. Therefore, targeting nodes crosstalk between is a potential strategy for cancer therapy. Melanoma the most lethal skin accounting over 80% deaths. We previously identified tumor necrosis factor receptor-associated 6 (TRAF6) was overexpressed melanoma involved malignant phenotype cells. Additionally, TRAF6 promotes activation fibroblasts to cancer-associated melanoma. However, role remains unclear. In this study, we found that knockdown significantly induced both percentage apoptotic cells elevated nearly three more times after knocking down TRAF6. Moreover, lots autophagosomes were observed TRAF6-deficient Then analyzed transcriptomics data decrease related 16 like 2 (ATG16L2) Real-time PCR further verify alteration. Interestingly, ATG16L2 resulted To investigate mechanism regulating ATG16L2, luciferase assay chromatin immunoprecipitation performed. confirmed inhibited abundance c-Jun nucleus, which suppressed transcription activity ATG16L2. Next, cinchonine, inhibitor, vitro vivo studies suggested cinchonine growth Further indicated TRAF6/c-Jun/ATG16L2 signaling pathway. Our suggest axis promising
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2023
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2023.03.1236